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KMID : 0624620190520050324
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BMB Reports
2019 Volume.52 No. 5 p.324 ~ p.329
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Enhanced delivery of protein fused to cell penetrating peptides to mammalian cells
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Moon Jung-Il
Han Min-Joon
Yu Shin-Hye
Lee Eun-Hye
Kim Sang-Mi
Han Kyu-Boem
Park Chang-Hwan
Kim Chun-Hyung
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Abstract
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Recent progress in cellular reprogramming technology and lineage-specific cell differentiation has provided great opportunities for translational research. Because virus-based gene delivery is not a practical reprogramming protocol, protein-based reprogramming has been receiving attention as a safe way to generate reprogrammed cells. However, the poor efficiency of the cellular uptake of reprogramming proteins is still a major obstacle. Here, we reported key factors which improve the cellular uptake of these proteins. Purified red fluorescent proteins fused with 9xLysine (dsRED-9K) as a cell penetrating peptide were efficiently delivered into the diverse primary cells. Protein delivery was improved by the addition of amodiaquine. Furthermore, purified dsRED-9K was able to penetrate all cell lineages derived from mouse embryonic stem cells efficiently. Our data may provide important insights into the design of protein-based reprogramming or differentiation protocols
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KEYWORD
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Amodiaquine (AQ), Cell Penetrating Peptide (CPP), Differentiation, Polylysine (9K), Reprogramming
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